DNA blotting

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• DNA fingerprinting — DNA fingerprint. the use of a DNA probe for the identification of an individual, as for the matching of genes from a forensic sample with those of a criminal suspect. Also called genetic fingerprinting. [1985 90] * * * Method developed by the… …   Universalium

• Blotting — Als Blotting oder Blotten bezeichnet man in der Molekularbiologie ein Verfahren zum Transfer von DNA, RNA oder Proteinen auf eine Membran. Der Name leitet sich aus dem englischen „to blot“ (klecksen, beflecken, mit Löschpapier abtupfen) ab, was… …   Deutsch Wikipedia

• DNA methylation — Illustration of a DNA molecule that is methylated at the two center cytosines. DNA methylation plays an important role for epigenetic gene regulation in development and disease. DNA methylation is a biochemical process that is important for… …   Wikipedia

• Blotting — Blọt|ting [engl. blot = [be]klecksen, ablöschen]: eine molekularbiol. Nachweismethode, bei der z. B. elektrophoretisch getrennte DNA Fragmente – nach ↑ Denaturierung (1) innerhalb der Gelschicht – auf eine Cellulosenitrat Matrix übertragen… …   Universal-Lexikon

• DNA microarray — A DNA microarray (also commonly known as gene chip, DNA chip, or biochip) is a collection of microscopic DNA spots attached to a solid surface. Scientists use DNA microarrays to measure the expression levels of large numbers of genes… …   Wikipedia

• blotting — General term for the transfer of protein, RNA or DNA molecules from a relatively thick acrylamide or agarose gel to a paper like membrane (usually nylon or nitrocellulose) by capillarity or an electric field, preserving the spatial arrangment.… …   Dictionary of molecular biology

• Gentechnik: Zerschneiden und Verbinden von DNA —   Gentechnologie ist in erster Linie DNA Technologie. Die Gene aller Lebewesen bestehen aus DNA. Die gezielte Veränderung von Genen setzt voraus, dass ein Gen beziehungsweise eine DNA gezielt zerlegt und wieder neu zusammengesetzt werden kann.… …   Universal-Lexikon

• Branched DNA assay — In biology, a branched DNA assay is a test for specific nucleic acid chains typically used to detect Retroviruses such as HIVcite journal author = Murphy, D. G.; Gonin, P.; Fauvel, M. year = 1999 title = Reproducibility and Performance of the… …   Wikipedia

• electro-blotting — The electrophoretic transfer of DNA, RNA or protein from a gel, in which they have been separated, to a support matrix, such as nitrocellulose. A transfer technique employed in Southern and northern blotting …   Glossary of Biotechnology

• Southern blotting technique — The procedure used to isolate and identify DNA fragments from a complex mixture. The isolated, denatured fragments are transferred from an agarose electrophoretic gel to a nitrocellulose filter and identified by hybridization with probes …   Dictionary of microbiology

• Southern blotting —    A technique developed by E. Southern for the direct transfer of DNA fragments from an agarose gel onto a solid support such as a nylon membrane. The transfer occurs by salt–solution capillary action …   Forensic science glossary